Projects
Generation and identification of antimicrobial species from medicines exposed to laser radiation in view of fighting multiple drug resistance acquired by bacteria
Project code: PN-III-P1-1.1-PD-2016-1072
Contract number:68PD/2018
Founded by: MCI, UEFISCDI
Program: Postdoctoral research projects
Contractor:
National Institute for Laser, Plasma and Radiation Physics
Project Leader: Dr. Tatiana Tozar
Start date: 02.05.2018
End Date: 30.04.2020
Stage 1: Irradiation experimental set-ups development and optical characterization of irradiated thioridazine and ciprofloxacin. Susceptibility test of
Gram-positive/-negative bacteria to irradiated TZ and CIP. (May 2018- December 2018)
Objectives:
- Update the concept of the project to last minute published progress in order to adapt the research to reaching the aims specified in the project proposal.
- Mount and test the experimental set-ups for irradiation and LIF experiments.
- Perform experiments using the developed systems on TZ and CIP.
- Optical characterization of the samples: UV-VIS and FTIR absorption, LIF and LIF lifetime measurements.
- Selection of Gram-positive/-negative bacteria sensible and resistant to CIP.
- Susceptibility test of Gram-positive/-negative bacteria to unirradiated and irradiated TZ and CIP – one international research stage and one national research stage.
- Selection of the best irradiation conditions in order to obtain agents with enhanced photo-antimicrobial effect that are going to be investigated with chromatographic techniques.
Deliverables: *technical documentation containing the working and irradiation parameters for the experimental set-up and solutions; *2 articles in peer-reviewed journals AIS>0.5; *2
scientific communications – international conference; *one international research stage, *one national research stage, *project website; *stage report.
Stage Report
Stage 2: HPTLC densitometry experimental set-up development and validation. : HPTLC densitometry assay on selected irradiated TZ solutions. Identification of the photo-products (January 2019-December 2019)
Objectives:
- Design, mount and test the experimental set-up for HPTLC densitometry - LIF and LIF lifetime assays.
- Establishing the linearity, precision and accuracy on TZ and CIP unirradiated solutions at different concentrations by LIF and LIF lifetime measurements.
- Analyze and perform quantitative measurements of the plates using JustTLC software.
- LC-TOF/MS experiments on TZ and CIP unirradiated solutions – one national research stage.
- Compare the results obtained with JustTLC software, HPTLC-densitometry and LC-TOF/MS and validate the method.
- Experiments using the HPTLC developed system on irradiated TZ, those for which the best antimicrobial effect was obtained.
- LC-TOF/MS experiments on selected irradiated TZ solutions.
- Determine the molecular structure of the photo-products resulted from TZ irradiation.
Deliverables: *2 article in peer-reviewed journals AIS>0.5; *2 scientific communications – international conference; * one national research stages; *project website–update; *stage report.
Stage Report
Stage 3: HPTLC densitometry assay on selected irradiated CIP solutions. Identification of the photo-products (January 2020-April 2020)
Objectives:
- Experiments using the HPTLC developed system on irradiated CIP, those for which the best antimicrobial effect was obtained.
- LC-TOF/MS experiments on selected irradiated CIP solutions.
- Determine the molecular structure of the photo-products resulted from CIP irradiation.
Deliverables: *national research stage *project website-update; *stage report; *final scientific report.
Stage Report
